show Abstracthide AbstractChina is the origin and evolution centre of Oriental pears. Pyrus betulaefolia is a wild species native to China, distributed in its northern region and widely used as rootstock. Here, we report the de novo assembly of the genome for P. betulaefolia 'Shanxi Duli 1' using an integrated strategy combining PacBio sequencing, BioNano mapping, and Hi-C mapping. The genome assembly size is 532.7 Mb, with a contig N50 of 1.57 Mb. A total of 59,552 protein-coding genes and 247.4 Mb of repetitive sequences were annotated for this genome.